23 Jul Bioreactor for constructing tissue engineering liver by using in-vitro circulatory perfusion
Abstract
The invention relates to a three-dimensional tissue culture device and a three-dimensional tissue culture method. The bioreactor comprises an in-vitro perfusion circulating system, a negative pressure feedback regulation system, a perfusion flow control module and a liver tissue culture module, wherein the in-vitro perfusion circulating system comprises a closed pipeline system, a tissue perfusion generator and a perfusate suspension update device; the negative pressure feedback regulation system comprises an internal pipeline pressure monitoring system, a computer control module and a perfusion device controlled and regulated by a computer; and the liver tissue culture module comprises a constructed sterile culture room and a constructed three-dimensional tissue culture scaffold. The in-vivo environment can be simulated, a device is provided for culturing tissue engineering organs in vitro, and a tool is provided for research of stem cell differentiation and cell growth under three-dimensional conditions.
Year: 2015
Country: CN
Doc No: 104611225