23 Jul Method for extracting mesenchymal stem cells from human umbilical cord
Abstract
The invention relates to the fields of stem cells and tissue engineering, in particular to a method for extracting mesenchymal stem cells from the human umbilical cord. To solve the problems and defects in the conventional separation and culturing technology for human umbilical cord mesenchymal stem cells, the method adopts the technical scheme that the separation method for the human umbilical cord mesenchymal stem cells comprises the following steps: 1. preparation of an umbilical cord tissue block; 2. digestion of the tissue block; 3. filtration of a digestion solution, and centrifugal cell collection; 4. combination of cell sediments, centrifugation, and cell collection; 5. subculturing; 6. cell cryopreservation through a programmable cooler, and cryopreservation in liquid nitrogen. The method provided by the invention can lower the cost, achieve rapid obtaining of the mesenchymal stem cells, reduce cell damages, prolong the cryopreservation period, improve the cell activity, shorten the operational process, reduce pollution, and improve the cell purity.
Year: 2015
Country: CN
Doc No: 104673748