23 Jul Acellular matrix with natural level of glycosaminoglycan and preparation and application thereof
Abstract
The invention discloses an acellular matrix with natural level of glycosaminoglycan and preparation and application thereof. The preparation method comprises the following steps: carrying out acellular disposal on natural biological tissue to obtain an acellular matrix; detecting missing amount of glycosaminoglycan in the acellular matrix; and supplementing glycosaminoglycan by means of natural ingredients in the acellular matrix to obtain the acellular matrix with natural level of glycosaminoglycan. Through the method, a lot of the glycosaminoglycan component in the acellular matrix is recovered. The acellular matrix has good biocompatibility. The natural biological structure is recovered, and the acellular matrix is beneficial to cell growth. glycosaminoglycan is supplemented for the acellular matrix, and a tissue engineering carrier is constructed. According to the invention, a biological carrier which meets cell growth requirement and mechanical strength requirement and has similar structural components as a natural extracellular matrix can be prepared rapidly. The invention is a great breakthrough of the tissue engineering construction technology. Meanwhile, principle of the invention is reliable, the technology is simple and flexible, product reproducibility is good, and industrialization is easy to realize.
Year: 2015
Country: CN
Doc No: 104888274