23 Jul Corn endosperm specific expression promoter PMAP promoter and cloning method and application thereof
Abstract
The invention relates to a corn endosperm specific expression promoter PMAP promoter and a cloning method and application thereof. The plasma membrane associated protein (PMAP) promoter capable of being specifically expressed in corn endosperm is separated out for the first time. The promoter has a nucleotide sequence shown as SEQ ID NO.1 or a nucleotide sequence which is replaced with, is in lack of and/or is added with one or more nucleotides, has the same function and is derived from the nucleotide sequence. Starting from cloning the corn endosperm specific expression promoter PMAP promoter, the function of the PMAP promoter is analyzed, and it is verified from the experiment level that the promoter is suitable for starting a target gene to be specifically expressed in corn endosperm, and is low expressed or not expressed in other tissue. A proper control element is provided for construction of a plant genetic engineering expression carrier, and new materials are provided for study on other projects in a laboratory.
Year: 2016
Country: CN
Doc No: 105316338