23 Jul Fast culturing method for lily virus-free bulblets
Abstract
The invention discloses a fast culturing method for lily virus-free bulblets; the fast culturing method comprises the steps: lily bulblets are thermally treated and planted for 7-15 days under a condition of the temperature of 35-38 DEG C; the stem tip growing points of the thermally treated lily bulblets are peeled to be taken as explants; a culture medium with the ribavirin content of 5-10 mg/L is inoculated with the explants, and virus-free cultivation is performed; and viruses, such as lily symptomless virus (LSV), cucumber mosaic virus (CMV) and lily mottle virus (LMoV) of virus-free tissue culture seedlings are detected by an enzyme-linked immunosorbent assay test (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) detection. The fast culturing method for the lily virus-free bulblets ensures that the cultured lily bulblets have the virus infection removed through the comprehensive virus-free technique; the tissue culture medium components and culture program of the virus-free lily bulblets are optimized; the virus-free tissue culture bulblets are obtained through fast culture; and the fast culturing method belongs to the technical field of plant tissue culture rapid propagation and the technical field of plant cell engineering.
Year: 2016
Country: CN
Doc No: 105613283