23 Jul Method for accelerating expanding culture of lily virus-free cormels
Abstract
The invention discloses a culturing method for utilizing a bubbling type bioreactor to quickly expand cut-flower lily virus-free bulbs (cormels), and belongs to the technical field of plant tissue culturing fast propagation and the field of plant cell engineering. The method comprises the following steps: optimizing an expanding liquid culture medium (MS+0.2 mg/L NAA +4 mg/L CPPU +6% cane sugar, wherein the pH value is 6.8) for tissue cultured lily virus-free cormel bulbs used for a reactor; planting cormel blocks according to the quantity of 2600-3000 cormel blocks per liter; adjusting an air inlet volume of filtered air to enable a flow speed of a turbulent area in the reactor to be 3-6 cm/s; keeping the pressure in the reactor to be constant at 0.03-0.06 MPa at the temperature of 22+/-1 DEG C; and irradiating 12 h/d under a luminous intensity of 1600-2000 Lx and culturing for 40-46 d so as to meet field planting demands on diameters, root systems and weight of the cultured cormels. According to the method, the culturing period can be shortened, the energy source is saved, the labor and culturing space are saved, the utilization efficiency of the culture medium is increased, the production cost is lowered, the practicability is strong and the method is easily promoted and applied in production.
Year: 2016
Country: CN
Doc No: 105613284