23 Jul Microfluidic chip-based tumor brain metastasis model building method and use
Abstract
The invention provides a microfluidic chip-based tumor brain metastasis model building method and use and belongs to the technical field of cytobiology and tissue engineering. The microfluidic chip comprises a waste liquid outlet, a cell inlet and a collagen inlet. The waste liquid outlet is communicated with the cell inlet through a two-dimensional passage. The collagen inlet is communicated with a three-dimensional passage. The junction of the two-dimensional passage and the three-dimensional passage forms a two dimensional-three dimensional microenvironment interface. The brain metastasis model building method utilizes the microfluidic chip and comprises 1, building a blood-brain barrier model through collagen perfusion into the chip and cell perfusion and culture on the chip, and 2, building a tumor brain metastasis model which is used for research on intracerebral metastasis of exogenous tumor cells and has characteristics of high throughput and high integrated level.
Year: 2016
Country: CN
Doc No: 105624036