23 Jul THREE-DIMENSIONAL STRUCTURE FOR CARDIAC MUSCULAR TISSUE REGENERATION AND MANUFACTURING METHOD THEREFOR
Abstract
Provided are a method for manufacturing a three-dimensional structure for cardiac muscular tissue regeneration, and a three-dimensional structure for cardiac muscular tissue regeneration, the method comprising the steps of: forming a three-dimensional structure by printing and crosslinking with a first bioprinting composition and a second bioprinting composition so as to alternately arrange a first bioprinted layer and a second bioprinted layer, wherein the first bioprinting composition contains cardiac progenitor cells and a tissue engineering structure formation solution containing decellularized extracellular matrix and a crosslinking agent, and the second bioprinting composition contains a tissue engineering structure formation solution, mesenchymal stem cells, and a vascular endothelial growth factor; and obtaining a crosslinked-gelled three-dimensional structure by performing thermal gelation on the crosslinked three-dimensional structure. The manufacturing method according to the present invention can uniformly position cardiac progenitor cells in the structure and maintain the viability of cells for a long time by implementing a blood vessel network composed of vascular cells in the structure, thereby remarkably improving the efficiency of cellular delivery into the myocardium.
Year: 2016
Country: WO
Doc No: 2016153320