23 Jul Preparation method of copper-free click crosslinking polysaccharide microspheres
Abstract
The invention discloses a preparation method of copper-free click crosslinking polysaccharide microspheres. The preparation method comprises the following steps that chitosan and 1-hydroxyl benzotriazole are dissolved in water at room temperature, and cyclooctyne-3-glycolic acid is dissolved in a tetrahydrofuran/water mixed solvent; after the two solutions are mixed, diisopropylethylamine is added, stirring reaction is performed, and cyclooctyne chitosan is obtained through dialysis and freeze-drying; sodium alginate and carbodiimide are dissolved in water, 11-azido-3,6,9-triethyl ether-1-amine is added, stirring reaction is performed, and azido sodium alginate is obtained through dialysis and freeze-drying; water solutions of the cyclooctyne chitosan and the azido sodium alginate are respectively prepared, after mixing according to the volume ratio, emulsifier Span-80 containing paraffin oil is added, and an emulsification emulsion is obtained through ultrasonic emulsification; the emulsification emulsion volatilizes and stays overnight and is poured into isopropanol for precipitation of microspheres, and the crosslinking polysaccharide microspheres are obtained through freeze-drying after cleaning. The process is simple, makes products safe and non-toxic and is suitable for the medical fields of drug release, gene therapy, tissue engineering and the like.
Year: 2016
Country: CN
Doc No: 106140040