23 Jul METHOD FOR CONDITIONING STEM CELLS
Abstract
There is provided a method of stimulating an isolated stem cell comprising two stimulation steps: (a) cell mechanical stimulation which comprises the application, to a culture medium comprising the isolated stem cells, of one or more mechanical pulses having a stretching value comprised from 5% to 15%, and a duration of time comprised from 300 to 700 ms, at a frequency comprised from 0.5 to 1.5 Hz; and (b) cell electrical stimulation which comprises the application, to a culture medium comprising the isolated stem cells, of one or more electrical pulses having a potential value comprised from 0mv/cm to 1000mv/cm, and a duration of time comprised from 1 to 3 ms, at a frequency comprised from 0.5 to 1.5 Hz. There is also provided the cell obtainable by the method, further products comprising the cell such as solutions and scaffolds, and its uses in tissue engineering and regenerative therapy.
Year: 2017
Country: WO
Doc No: 2017125159