23 Jul Cell cross-linking agent material for three-dimensional hepatocyte sphere culture
Abstract
The invention discloses a cell cross-linking agent material for three-dimensional hepatocyte sphere culture. The synthetic hepatocyte cross-linking agent material provided by the invention comprises ALG-GA-N(CH3)2 and SCTS-GA-N(CH3)2. Sodium alginate (ALG) or sulfonated chitosan is used as a carrier, and sodium alginate (ALG) is modified through glycyrrhetinic acid (GA-N(CH3)2) subjected to hydrophilic modification through ethylenediamine as a connecting arm or the modified glycyrrhetinic acid directly modifies the sulfonated chitosan. A cell co-culture experiment research proves that through simple mixing of the ALG-GA-N(CH3)2 or SCTS-GA-N(CH3)2 and hepatocytes, cell aggregation is promoted and three-dimensional hepatocyte spheres are formed, and the three-dimensional hepatocyte spheres have physiological functions better than those of the two-dimensional cultured hepatocytes. The cell adhesion material provided by the invention can be used for constructing a three-dimensional cell model and has a good application prospect in the fields of tissue engineering and drug research and development.
Year: 2017
Country: CN
Doc No: 106399228