23 Jul Fibroblast culture medium and preparation method thereof
Abstract
The invention relates to a culture medium by which induced pluripotent stem cells are induced into fibroblasts and a preparation method of the culture medium. The culture medium consists of a basic culture solution and an additive, wherein the basic culture solution is a mixture of an H-DMEM culture solution and an F12 culture solution at the volume ratio of 1: (0.5-2); and in terms of concentrations in the basic culture solution, the additive consists of the following components: nonessential amino acid, glutamine derivatives, insulin, hydrocortisone, transferrin, sodium selenite, fibroblast growth factors, estradiol, progestin, 8-bromo cyclophosphate and human follicle stimulating hormone. The culture medium, in comparison with the prior art, is higher than fibroblast differentiation probability; in addition, the culture medium can guarantee stable passage and improve cell propagation speed, so as to offer a fibroblast seed cell source for skin tissue engineering; and meanwhile, the application of serum is avoided in an entire course, so that risks caused by animal-derived pathogens are effectively avoided and the safety of clinical application is enhanced.
Year: 2017
Country: CN
Doc No: 106635972