23 Jul Method for building tissue engineering limbus corneae
Abstract
The invention relates to the field of bioengineering medical materials and discloses a method for building tissue engineering limbus corneae. The method comprises the following steps of putting fresh pig corneoscleral limbus matrix containing a lamina elastica anterior into a decellularized limbus corneae protecting liquid added with a detergent and nuclease for oscillating acellular treatment; putting decellularized limbus corneae into the decellularized limbus corneae protecting liquid for rinsing to obtain the decellularized limbus corneae; inoculating seed cells into the decellularized limbus corneae for amplification in vitro; obtaining the tissue engineering limbus corneae when the seed cells are amplified to 3-5 layers on the decellularized limbus corneae, wherein the decellularized limbus corneae protecting liquid is a PBS buffer solution containing 5-12g/L hyaluronic acid, 5-20g/L chondroitin sulfate, 3-10g/L dextran and 2.5-5mg/L L-tobramycin. Human limbus corneae can be replaced with the tissue engineering limbus corneae material.
Year: 2017
Country: CN
Doc No: 106729999