23 Jul Method for preparing gracilaria chilensis protoplast
Abstract
The invention relates to a method for preparing gracilaria chilensis protoplast and belongs to the field of algae cell engineering. The preparation method comprises the following steps: preparing a tissue enzyme solution, and performing enzymolysis on gracilaria chilensis tissues, wherein the step of preparing the tissue enzyme solution comprises the following steps: homogenizing with seawater in an amount of 1-10 times that of the volume of sea cucumber intestines at a temperature of 0 DEG C; extracting at a temperature of 4 DEG C for 4-18 hours, regulating the pH value of the extracting solution to be 7.0-8.0, freezing and centrifuging for 5 minutes, and taking the supernatant so as to obtain a sea cucumber enzyme solution; and mixing the sea cucumber enzyme solution, cellulase and seawater so as to obtain the tissue enzyme solution.
Year: 2017
Country: CN
Doc No: 106893690