23 Jul Preparation method of gelatin sponge scaffold
Abstract
The invention discloses a gelatin sponge scaffold. A preparation method of the gelatin sponge scaffold comprises the following steps: (1) preparing gelatin solution; (2) preparing transglutaminase solution; and (3) mixing the gelatin solution prepared in the step (1) and the transglutaminase solution prepared in the step (2), carrying out incubating, thus forming hydrogel, carrying out refrigerating, and carrying out freeze drying, thus preparing the sponge scaffold. The sponge scaffold is free of cytotoxicity, and has excellent physical property, excellent comprehensive performance, good porosity, good compression modulus, and good degradation resistance; in addition, the biocompatibility is good, so that cells can well grow in the scaffold; the scaffold is implanted into the body, a formed material wrapping layer is thin, blood capillaries are formed on the outer wrapping layer, and thus the growth of the regenerated tissue is facilitated; the gelatin sponge scaffold can be taken as the materials such as the tissue engineering scaffold, clinical hemostatic sponge and medicine controlled-release carriers to be used for the cell and tissue engineering applications, and has the broad prospect.
Year: 2017
Country: CN
Doc No: 107261216