23 Jul Preparation method of microcarrier used for cell three dimensional multiplication culture
Abstract
The invention discloses a preparation method of a microcarrier used for cell three dimensional multiplication culture, and belongs to the field of tissue engineering material. According to the preparation method, Poly-L-lactic acid (PLLA) is taken as a matrix, gelatin is taken as a dispersant, and composite microspheres are prepared; the particle size of the composite microspheres is adjusted viacontrolling the concentration of a PLLA dichloromethane solution, the mass ratio of nano-hydroxyapatite (nHAp) to PLLA, and the using amount of gelatin; the PLLA is taken as the matrix to ensure the mechanical strength and biological degradability of a support; composite nHAp is capable of improving the osteo inductivity and the conductivity of the support, and at the same time, assisting PLLA toimprove cell osteogenic capability, and avoiding damage of cells under an overly acidic environment; the existing of nHAp is capable of forming a large amount of small pores on the surfaces of the PLLA microspheres, the existing of the small pores is capable of improving the mass transfer capability of the support, and providing cells with more abundant attachment sites. The microcarrier possessesexcellent biological performance on osteoblasts, and is suitable to be used in the field of bone tissue engineering.
Year: 2018
Country: CN
Doc No: 107670113