23 Jul Preparation method of biogenic small-aperture tissue engineering blood vessels
Abstract
The invention relates to a preparation method of biogenic small-aperture tissue engineering blood vessels, and belongs to the fields of tissue engineering and biological materials. The preparation method of the biogenic small-aperture tissue engineering blood vessel comprises the following steps: (1) freeze thawing: placing fresh animal blood vessels into a PBS (Phosphate Buffer Solution) containing 0.02 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid), and performing quick freezing at the temperature of 80 DEG C below zero for 1 to 3 hours, taking the fresh animal blood vessels out,and immediately performing unfreezing at the temperature of 37 DEG C for 10 to 30 minutes; repeating the abovementioned operations for 2 to 5 times to obtain a blood vessel material; (2) removing cell debris fallen after the freeze thawing; (3) removing cells; (4) removing a detergent. The invention has the advantage that a cell removing method and a reagent composition which can effectively remove cell components in blood vessel tissues and protect the structural integrity of acellular matrixes to the maximum extent are provided.
Year: 2018
Country: CN
Doc No: 108144121