23 Jul Culture method for improving differentiation from peripheral blood multipotent cells to cartilage cells
Abstract
The invention relates to a culture method for improving differentiation from peripheral blood multipotent cells to cartilage cells, and belongs to the technical field of cytobiology and regenerative medicine. The culture method comprises the following steps: carrying out primary culture on peripheral blood multipotent mesenchymal matrix cells to obtain third-generation cells; co-culturing the third-generation passed-down peripheral blood multipotent mesenchymal matrix cells and human articular chondrocytes in an induction culture medium. According to the culture method for improving the differentiation from the peripheral blood multipotent cells to the cartilage cells, the peripheral blood multipotent stem cells and the chondrocytes are co-cultured and the two types of cells mutually promote proliferation and the differentiation; the number of times of chondrocyte proliferation and passage and the quantity of the chondrocytes can be reduced, and the differentiation is improved; an efficient method is provided for establishing a seed cell source, and a new medical experiment evidence is also provided for enlarging a cell source of cartilage tissue engineering.
Year: 2019
Country: CN
Doc No: 109112102