23 Jul Primary culture and serum-free multidimensional induced differentiation method of adipose-derived stem cells
Abstract
The invention belongs to the technical field of biology tissue engineering, and discloses a primary culture and serum-free multidimensional induced differentiation method of adipose-derived stem cells. A collagenase dissociation method is adopted for obtaining the adipose-derived stem cells from subcutaneous and omentum tissue, primary culture is performed until 80% is merged, subculturing is performed, and identification of the fat mesenchyme stem cells is performed. Third generation cells in favorable growth state are selected, a serum-free induced culture medium is added for forming fat andosteogenesis induced differentiation, and finally, oil red O dying and alizarin red dying are performed for identifying the differentiation effect and Real-time RCR is used for detecting the situation of fat formation and osteogenesis gene expression. The adipose-derived cells are easy to obtain, and through primary culture, the fat mesenchyme stem cells are adequate in survival volume and survival period, and ripe lipoblast and osteoblast can be formed through induced differentiation. The primary culture and serum-free multidimensional induced differentiation method provides ideal seed cellsfor cell treatment of metabolic diseases of diabetes, obesity, osteoporosis diseases and the like. A new way is provided for organizational project biolization bones and biolization fat, and the method has wide clinical application prospects.
Year: 2019
Country: CN
Doc No: 109837239