23 Jul Efficient preparation method of placental mesenchymal stem cells through homogenizer
Abstract
The invention relates to separation and purification of placental derived mesenchymal stem cells and belongs to the field of stem cell and tissue engineering. The efficient preparation method includes: with the consent of the parturient and her family, placing the placenta after caesarean birth of the parturient in a storage solution with a variety of antibioctics for a short period of time; crushing the placental after repeatedly cleaning blood and water of the placental in a laboratory, digesting by collagenase and patting with a homogenizer to obtain a cell suspension; using a density gradient centrifugation method to obtain the placental mesenchymal stem cells. The method for separating the placental derived mesenchymal stem cells with the homogenizer is multiple times higher than thatof traditional methods, the hydrolysis time is reduced, and the success rate is improved; the obtained mesenchymal stem cells have similar amplification capability and multifunctional differentiationpotential with bone marrow derived mesenchymal stem cells in vitro and are potential seed cell sources for tissue engineering and cell therapy, thereby having important clinical significance.
Year: 2019
Country: CN
Doc No: 110373381