23 Jul Method for constructing three-dimensional engineered nerve tissue, three-dimensional engineered nerve tissue and application thereof
Abstract
The invention relates to a method for constructing three-dimensional engineered nervous tissue, the three-dimensional engineered nervous tissue and an application thereof. Process. The method comprises the following steps: 1, carrying out isolated culture on animal neural stem cells; 2, applying a physiological electric field; subculturing the neural stem cells isolated and cultured in the step 1to the fifth generation, centrifuging neural spheres with a diameter of 100-200 microns for later use, pre-melting matrix gel Matrigel on ice for later use, and continuously applying a physiological electric field to stimulate for 7-14 days through physiological electric field application equipment for 1-2 hours every day; 3, optimizing a culture solution suitable for engineering nerve tissue growth: adding 1/50 of B27, 1/100 of N2, 10-20 ng/ml of bFGF and 1% of fetal calf serum into DMEM/F12; and 4, applying the physiological electric field for 7-14 days to obtain the three-dimensional engineered nervous tissue. The technical problems that in the application process of neural tissue engineering, the number of neuronal differentiation of existing neural stem cells is extremely small, and neurite cannot be induced to grow orderly in a three-dimensional growth environment to form a developed neural network are solved.
Year: 2020
Country: CN
Doc No: 110862966