23 Jul 3D printing preparation method of skin tissue engineering scaffold and in vitro cell toxicity testing method of the same
Abstract
The invention provides a 3D printing preparation method of a skin tissue engineering scaffold and an in vitro cell toxicity testing method of the scaffold. The 3D printing preparation method comprisesthe following steps: preparation of double aldehyde nano-crystalline cellulose used for a scaffold material, preparation of a gelatin solution, preparation of a double aldehyde nano-crystalline cellulose and gelatin compound hydrogel, and printing of the 3D tissue engineering scaffold. The problem that the tissue engineering scaffold needs the requirements of high porosity and high precision is solved by using a 3D biological printing technology. DAC is taken as a cross-linking agent, cross-linking occurs with GEL through a Schiff base reaction to form a network structure, so that the 3D printing tissue engineering scaffold has excellent mechanical property and is unlikely to crack, and simultaneously the additional value of plant fiber is also improved. The DAC/GEL hydrogel has good biocompatibility, is free of toxic and/or side effects or immunologic rejection, has biological activity besides degradation characteristic, and is extremely beneficial for the growth and differentiationof cells and the implementation of cell functions.
Year: 2018
Country: CN
Doc No: 107998451