23 Jul 3D multi-layer high-flux organ chip and biological barrier model constructed based on 3D multi-layer high-flux organ chip
Abstract
The utility model discloses a 3D multi-layer high-flux organ chip and a biological barrier model constructed based on the same, and belongs to the field of biological tissue engineering. The chip comprises a liquid storage layer, a thin film layer and a 3D culture layer which are stacked in sequence. And constructing a vascular endothelial cell layer on the thin film layer on the liquid storage layer side of the chip, and carrying out 3D culture on cells in the culture micropores of the 3D culture layer of the chip to construct the biological barrier model. The chip can be used for researchinginteraction of two cells, angiogenesis, drug permeation, cell polarization, cell migration, drug activity evaluation and the like. Moreover, an in-vitro model and a biological barrier model which arecombined with high-throughput drug screening can be constructed, and the method is used for screening of anti-brain glioma compounds of blood-brain barriers and related research. Moreover, the liquidis convenient to operate and suitable for high flux, and external equipment is not needed.
Year: 2020
Country: CN
Doc No: 210103904