23 Jul 3D hepatoma tissue construction method based on biological 3D printing hydrogel
Abstract
The invention discloses a 3D hepatoma tissue construction method based on biological 3D printing hydrogel. The method comprises the steps as follows: 1) a GelMA biomaterial is synthesized, and the acylation rate and light polymerization time of the GelMA biomaterial are detected; 2) after GelMA and an photoinitiator are uniformly mixed and dissolved, human hepatoma cell HepG2 and GelMA solutions are uniformly mixed, the cell concentration is adjusted to 1*10<6>/mL, and biological printing ink is prepared. Biological printed cells and a hydrogel system are placed under an ultraviolet lamp for light polymerization for 5 min to be cured, the cured system is placed in a 10%FBS culture solution to be cultured for 7 d, and a hepatoma tissue engineered unit containing an HepG2 source is prepared and saved for later use; 3) the swelling ratio and balance water content of a 3D hepatoma tissue material are detected; 4) the growth states of 2D and 3D on the seventh day are detected with a BE histochemistry method. The method is reliable, convenient to operate and high in repeatability and can effectively promote formation of hepatoma tissue functions and provide beneficial reference for scientific research of hepatic tissue engineering and clinical treatment of hepatoma.
Year: 2017
Country: CN
Doc No: 107400661